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His-Tag Protein Purification theory & practice - Immobilized Metal Affinity Chromatography (IMAC)
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YouTubethe bumbling biochemist
His-Tag Protein Purification theory & practice - Immobilized Metal Affinity Chromatography (IMAC)
IMAC (Immobiolized Metal Affinity Chromatography) is a way to help purify proteins that we’ve added a “His tag” onto to make them specifically sticky to resin (little beads) that are coated in a metal such as nickel (Ni) or cobalt (Co). So when we express a protein with a His tag then break open the cells (lyse them) and flow all that ...
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